Radiation risk assessment in professionals working in dental radiology area using buccal micronucleus cytome assay.
نویسندگان
چکیده
OBJECTIVE The aim of this study was to assess the incidence of micronuclei (MN) in buccal mucosal cells of professionals working in radiology area to determine the risk of stochastic effects of radiation. MATERIALS AND METHODS All the professionals and students working in King Khalid University - College of Dentistry radiology area were included in the Risk Group (RG = 27). The Control Group (CG = 27) comprised of healthy individual matching the gender and age of the RG. Buccal mucosal scraping from all the 54 subjects of RG and CG were stained with Papanicolaou stain and observed under oil immersion lens (×100) for the presence of micronuclei (MN) in the exfoliated epithelial cells. RESULTS There was no significant difference between the incidence of MN in RG and CG (p = >0.05) using t-test. CONCLUSION Routine radiation protection protocol does minimize the risk of radiation induced cytotoxicity, however, screening of professionals should be carried out at regular intervals.
منابع مشابه
Micronucleus Investigation in Buccal Mucosal Cells of Young Waterpipe Tobacco Smokers in Tehran
Waterpipe Tobacco Smoke(WTS) is an unhealthy life style that may increase the risk of genotoxic responses and chronic diseases such as cancer. Micronucleus test is a successful and reliable method which is used for screening of genetotoxic responses of whole body and also for screening those people who had already exposed to genotoxic compounds. In this study, specific questionnaires were desig...
متن کاملMicronucleus Assay in Exfoliated Buccal Epithelial Cells Using Liquid Based Cytology Preparations in Building Construction Workers
Background and objective:Cytogenetic damage in exfoliated buccal epithelial cells due to environmental and occupational exposure is often monitored by micronucleus (MN) assay using liquid based cytology (LBC) preparations. This study was performed to evaluate MN in exfoliated buccal epithelial cells of building construction workers using LBC preparations. <strong...
متن کاملEvaluation of Micronucleus Frequency and Related Factors in the Buccal Mucosa of Cone Beam Computed Tomography-Exposed Subjects
Background and aim: The ionizing radiation has been recognized to have deleterious effects on the DNA and induce cell death. Due to the importance of evaluating the DNA alterations in buccal mucosa cells induced by the ionizing radiation and by considering the limited volume of the samples used in previous studies, the present study aimed to assess the micronucleus (MN) incidence in the buccal ...
متن کاملWorkers exposed to wood dust have an increased micronucleus frequency in nasal and buccal cells: results from a pilot study.
Wood dust is recognised as a human carcinogen, based on the strong association of wood dust exposure and the elevated risk of malignant tumours of the nasal cavity and paranasal sinuses [sino-nasal cancer (SNC)]. The study aimed to assess genetic damage in workers exposed to wood dust using biomarkers in both buccal and nasal cells that reflect genome instability events, cellular proliferation ...
متن کاملDifferent aspects of cytochalasin B Blocked micronucleus cytome (CBMN cyt) assay as a comprehensive measurement tool for radiobiological studies, biological dosimetry and genome instability
It is now universally accepted that DNA is the main target for damages caused by physical and chemical genotoxicants. Although there are different methods to measure directly the induced DNA damages but due to fast repair processes in cellular environment, most of the damages would be repaired even before sampling, therefore processed DNA damages, i.e. damages left unrepaired after acting repai...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The journal of contemporary dental practice
دوره 14 6 شماره
صفحات -
تاریخ انتشار 2013